Validation of RIC8A expression in cell lines by qRT-PCR. Relative expression level of RIC8A measured by TaqMan qRT-PCR in treated vs. non-treated cell lines is shown with black and grey bars, respectively. Each sample was run in triplicate and the relative gene expression levels were determined with the standard curve method and normalized using PGK1 as reference gene.
Muggerud et al. BMC Medical Genomics 2009 2:26 doi:10.1186/1755-8794-2-26