Additional File 3.

Bisulfite-sequencing assay (BSA) and Methylation-specific PCR (MSP). A) Demostration of NDRG2 promoter methylation by bisulfite-sequencing from: in vitro methylated DNA (IVD), normal lymphocytes (NL), CaCo2 cell line, normal (N) and tumour (T) tissue of one patient. Note methylation of 4 depict CpG islands (CpG sites 13–16). B) Methylation-specific PCR of NDRG2 gene in two colon cancer cell lines (HCT116 and CaCo2), in normal lymphocyte (NL) and in vitro methylated DNA (IVD). U, primers specific for unmethylated DNA; M, primers specific for methylated DNA.

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Piepoli et al. BMC Medical Genomics 2009 2:11   doi:10.1186/1755-8794-2-11