Stability of reference gene expression in RNA isolated from FF and FFPE material. Raw Cts are shown for 9 putative reference genes (ACTB, GAPDH, GUSB, RPLP0, TFRC, RPL7A, RPS11, RPS23 and UBB). Results based on intact RNA derived of FF material (A) and based on RNA isolated according to our own protocol from FFPE material (B) are depicted for all the 14 tumors. The Ct values for 4 ER-related genes (BCL2, CEPG1, ESR1 and PGR) are shown for comparison (left).
Oberli et al. BMC Medical Genomics 2008 1:9 doi:10.1186/1755-8794-1-9