Reproducibility of RNA isolation from FFPE material. The RNAs were isolated from paraffin blocks according to our own protocol. BM33 and BM36 (panel A) are two separate RNAs isolated from tissue block "BM", D33 and D36 are RNAs isolated from block "D" (panel B). For comparison, 45T and 56T originate from two distinct tumors isolated from one patient (panel C). Gene expression was measured by QPCR for 24 genes and raw Ct values are shown for each gene measured from the two matching RNAs.
Oberli et al. BMC Medical Genomics 2008 1:9 doi:10.1186/1755-8794-1-9