Comparison of RNAs isolated according to different protocols. RNA was reverse transcribed in the presence of random primers (white boxes) or gene-specific primers (hatched boxes). Gene expression was measured from an equivalent of 4 ng of RNA by QPCR for five reference genes (GAPDH, GUSB, RPLP0, TFRC and UBB). Pearson correlations were computed between matched Cts for the five reference genes and each tumor RNA isolated from FF (A) and FFPE material. Shown are correlations between intact RNA and RNA isolated from FFPE material according to the RNeasy FFPE protocol (A versus B), intact RNA and RNA isolated from FFPE material according to the ncLysis system (A versus C) and intact RNA and RNA isolated according to our own protocol (A versus D).
Oberli et al. BMC Medical Genomics 2008 1:9 doi:10.1186/1755-8794-1-9