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This article is part of the supplement: São Paulo Advanced School of Comparative Oncology: Abstracts

Open Access Poster presentation

MicroRNAs from peripheral blood mononuclear cells as biomarkers for detection of preclinical fibrosarcoma

Adriane F Evangelista1, Danilo J Xavier2, Elza T Sakamoto-Hojo2, Eduardo A Donadi2, Geraldo A Passos2 and Marcia MCM Silveira1*

Author Affiliations

1 Molecular Oncology Research Center, Barretos Cancer Hospital, CEP:14784-400, Barretos, SP, Brazil

2 Molecular Immunogenetics Group, Department of Genetics, Faculty of Medicine, USP, Ribeirão Preto, SP, Brazil

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BMC Proceedings 2013, 7(Suppl 2):P2  doi:10.1186/1753-6561-7-S2-P2


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/7/S2/P2


Published:4 April 2013

© 2013 Evangelista et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Blood immune cells cooperate to prevent the progression of tumors through cancer immunosurveillance. Since activated peripheral immune cell clones trigger a sensitive transcriptional response upon recognition of tumors, which can be identified by transcriptional profiling, we hypothesised that peripheral blood mononuclear cells (PBMCs) could be used as reporters for cancer detection.

Materials and methods

We used a model system in which groups of immunocompetent BALB-c mice were subcutaneously injected with different numbers of tumorigenic B61 fibrosarcoma cells. The groups of study were: (i) tumoral group with serial injections of 102 to 106 cells; (ii) negative control group represented by sterile nonpyrogenic saline, (iii) inflammation group by Zymozan (Sigma) and (iv) bacterial infection group by injection of 107colony forming units [cfu] pool from mice feces. Mouse peripheral blood was collected three days after injection; blood samples (N=10) were pooled according to experimental conditions. Mononuclear cells were separated by centrifugation on a Ficoll-Hypaque cushion (GE Healthcare) and RNA was extracted using Trizol Reagent (Invitrogen). Samples were hybridizated on miRNA microarrays (Agilent).

Results

We identified four microRNAs, miR-451, miR-144, miR-486 and miR-494, which were differentially expressed when compared to control groups, including inflammation and bacterial infection.

Conclusions

Our results showed that PBMC microRNA expression profiling can serve as a sensitive method for detection of preclinical cancer.

Financial support

FAPESP.