Optimization of HEK 293 cell growth a) The effect of 10% FBS supplementation in commercially av ailable serum-free media, b) Growth kinetics of HEK 293 cells in 3 different serum-free protein-free formulations (bI, bII and bIII) in the presence or absence of a pre-defined mixture of supplements, c) Effect of recombinant proteins or synthetic lipid mix on HEK 293 cell growth, d) Response surface graphs. HEK 293 peak cell density as a function of the concentrations of Lipid Mix (X) vs. r-Transferrin (mg/L) (dI), r-Transferrin (mg/L) vs. r-Insulin (mg/L) (dII) and Lipid Mix (X) vs. r-Insulin (mg/L) (dIII) based on Box-Behnken experimental results. Cell density values presented are in millions of cells/mL and represent mean ± SD (n=3).
Cervera et al. BMC Proceedings 2011 5(Suppl 8):P126 doi:10.1186/1753-6561-5-S8-P126