Cloning medium choice does not impact clone titer distribution. The same Molecule 1-producing stable pools were seeded for limiting dilution cloning in either a lean cloning medium prototype or CD FortiCHOTM Medium. Following clone scale-up, the top clones from each process were assessed in shake flasks using a simple fed-batch process; day 7 data are shown. While there are differences in the absolute numbers, especially for the top 3 clones, overall the clones produced similarly whether they were isolated from lean cloning medium prototype or from CD FortiCHOTM Medium.
Sabourin et al. BMC Proceedings 2011 5(Suppl 8):P102 doi:10.1186/1753-6561-5-S8-P102