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This article is part of the supplement: IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery

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Isolation, characterization and genes expression analysis of three dehydrin genes during cold acclimation of Eucalyptus globulus

Marta Fernández1 and Sofia Valenzuela2

  • Corresponding authors: Marta Fernández - Sofia Valenzuela

  • † Equal contributors

Author Affiliations

1 Genómica Forestal S.A., Chile

2 Universidad de Concepción, Facultad de Ciencias Forestales. Centro de Biotecnología. Genómica Forestal S.A., Chile

BMC Proceedings 2011, 5(Suppl 7):P81  doi:10.1186/1753-6561-5-S7-P81

The electronic version of this article is the complete one and can be found online at:

Published:13 September 2011

© 2011 Fernández and Valenzuela; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Eucalyptus globulus is an important species for pulp production in Chile; however it has a high sensitivity to frost. During the last few years, many studies have directed their efforts to elucidate the molecular mechanisms that regulate plant response to cold stress through the analysis of gene expression.This work reports the isolation and characterization of coding and non coding sequence of three dehydrin genes of E. globulus which allowed comparing the gene expression of these genes during cold acclimation, the type of dehydrin and the presence of regulatory elements in their promoters which provided valuable information about the possible signaling pathways and regulation of these genes. The three dehydrins identified in E. globulus showed a high transcript accumulation in stem and leaf tissue of acclimated plants, compared to non-acclimated, and the highest transcript accumulation was observed after the exposition of plants to night frosts (-2°C). Furthermore, the freezing resistant genotype exhibited a higher transcript accumulation after frost exposition compared to the sensitive genotype. These results support the idea that dehydrin proteins have an important rol during cold acclimation and frost tolerance in E globulus.