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This article is part of the supplement: International Conference on Prevention & Infection Control (ICPIC 2011)

Open Access Poster presentation

Microbial load in instruments used in surgeries classified as clean

FMG Pinto1*, GADA Moriya12 and KU Graziano1

  • * Corresponding author: FMG Pinto

Author Affiliations

1 Departamento de Enfermagem Médico-Cirúrgica, Nursing School – University of Sao Paulo, Brazil

2 CSSD, Hospital Alemão Oswaldo Cruz, São Paulo, Brazil

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BMC Proceedings 2011, 5(Suppl 6):P313  doi:10.1186/1753-6561-5-S6-P313

The electronic version of this article is the complete one and can be found online at:

Published:29 June 2011

© 2011 Pinto et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction / objectives

The number of orthopedic surgery, especially surgery of total hip and knee, have been more frequent due to technological advances. This study aims to determine the microbial load in the instruments used in clean surgeries, quantifying and identifying the genus and species of microbial growth.


Orthopedic surgical instruments were immersed, after use, in sterile water, sonicated in ultrasonic washer and consecutively shaken. Then, the lavage was filtered through a 0.45micron membrane, the result was incubated in aerobic medium, anaerobic medium and medium for fungi and yeasts.


In clean surgeries, results showed that 47% of used instruments had microbiological growth in the range of 1 to 100 CFU/instrument. The most prevalent organism was Staphylococcus coagulase negative (28%), followed by Bacillus subtilis (11%).This study refuted the hypothesis that clean surgeries happen in micro-organismsfree surgery field.


The microbiological findings reinforce the importance of antibiotic prophylaxis, practice already well established for this category of surgical procedure.

Disclosure of interest

None declared.