Introduction / objectives
The infection associated to metal surfaces or dead tissues like bone grafts, can be fatal for the patient and presents a major financial burden for the economy. The adhering bacteria in these cases can evade host defences by forming biofilms. For this reason, the prevention of bacterial colonization and control of implant associated infections are of special interest.
Growth of S.aureus and S. epidermidis biofilms in vitro for antibiotic susceptibility tests and investigation of architecture.
S. aureus and S. epidermidis biofilms were grown over MBEC® (modified microtiter plates). Antibiotic susceptibility tests were carried out using gentamicin, vancomycin, rifampicin, fosfomycin, clindamycin and linezolid. Cell counting, opacity density (OD620) and scanning electronic microscopic (SEM) analysis were carried out.
The counting of viable cells after antibiotic exposition and OD620 showed significant efficacy of rifampicin and gentamicin against S. epidermidis biofilms and rifampicin against S. aureus biofilms compared to other antibiotics. SEM images showed proteic material in contact with cells which can be related to the proteic membrane characteristic of the biofilms structure.
The method for the development of bacterial biofilm in vitro using MBEC® plates is efficient and relatively fast. Gentamicin and rifampicin are good candidates for control of implant associated infections.
Disclosure of interest