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This article is part of the supplement: Proceedings of the International Symposium on Animal Genomics for Animal Health (AGAH 2010)

Open Access Proceedings

Toll-Like Receptor 6 differential expression in two pig genetic groups vaccinated against Mycoplasma hyopneumoniae

Katiene Régia Silva Sousa1, André Mauric Frossard Ribeiro1, Paulo Roberto Nunes Goes1, Simone Eliza Facioni Guimarães1*, Paulo Sávio Lopes1, Renata Veroneze1 and Eliane Gasparino2

Author Affiliations

1 Departamento de Zootecnia – Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brasil, 36570-000

2 Departamento de Zootecnia – Universidade Estadual de Maringá, Maringá, Paraná, Brasil, 87020-900

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BMC Proceedings 2011, 5(Suppl 4):S9  doi:10.1186/1753-6561-5-S4-S9

Published: 3 June 2011

Abstract

Background

Mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia, which causes important economic losses to swine industry. The Toll-like receptors (TLRs) are pattern-recognition receptors which detect microbial presence and initiate the innate as well as the adaptative immune defense. Toll-like receptor 6 is a type I transmembrane protein that recognizes bacterial components. The aim of this study was to compare mRNA expression pattern of TLR6 gene in two genetically distinct groups of pigs vaccinated against Mycoplasma hyopneumoniae.

Methods

For each genetic group, peripheral blood was collected just before and 10 days after vaccination from 10 Naturalized Brazilian Piau breed and 10 Commercial White Line serum-negative female piglets. RNA was extracted from peripheral blood mononuclear cells (PBMCs), reverse transcripted and the qRT-PCR performed using SYBR green fluorescence system, using GAPDH gene as endogenous control. Analyses were performed by UNIVARIATE (Shapiro-Wilk test) and MIXED procedures of SAS software (version 9.0).

Results

It was observed significant interaction between breed and vaccination, being the TLR6 mRNA expression higher in the Commercial White line than in the Piau breed after vaccination. Furthermore, there was differential expression before and after vaccination in the Commercial White line.

Conclusions

Analysis of in TLR6 gene expression showed difference between the two distinct genetic groups, however, other TLRs gene expression must be evaluated for a better understanding of innate resistance in the pig concerning Mycoplasma hyopneumoniae infection.