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This article is part of the supplement: Abstracts of the 16th International Charles Heidelberger Symposium on Cancer Research

Open Access Poster presentation

Metabolic responses of A549 lung cells to cisplatin and radiation exposure studied by 1H NMR spectroscopy

Ana F Ladeirinha12*, Inês D Lamego2, Ana M Gil2, Joana B Melo1, Isabel M Carreira13 and Iola F Duarte23

Author Affiliations

1 Cytogenetics Laboratory and CNC, Faculty of Medicine, University of Coimbra, Coimbra, Portugal

2 CICECO, Department of Chemistry, University of Aveiro, Aveiro, Portugal

3 CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, Portugal

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BMC Proceedings 2010, 4(Suppl 2):P42  doi:10.1186/1753-6561-4-S2-P42

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/4/S2/P42


Published:24 September 2010

© 2010 Ladeirinha et al; licensee BioMed Central Ltd.

Poster presentation

This work aims to characterize the dynamic metabolic responses of A549 lung tumor cells exposed to cisplatin (CDDP) and to 6 Gy ionizing radiation over a period of 48h. Control and CDDP/radiation treated cells, in the form of lysed suspensions, were directly analyzed by 1H High Resolution Magic Angle Spinning (HRMAS) NMR spectroscopy (500 MHz) and the changes in their intracellular metabolic profiles assessed by spectral integration and multivariate analysis. In this way, consistent variation patterns could be detected and specific metabolic effects related to drug and/or radiation exposure could be identified. In particular, significant time-dependent alterations were found in lipids and choline-containing compounds, as well as in low molecular weight metabolites such as some amino acids and nitrogenated bases. The results presented show that 1H NMR spectroscopy is a powerful tool for providing detailed biochemical information about the effects induced on cultured cells by external perturbations, such as a chemotherapy drug and ionizing radiation. In the future, this approach will be applied to lung cancer primary cultures subjected to different treatment regimens.