MBP-hNaa30p specificity constants (V/K) for selected oligopeptides based on detection of acetylated oligopeptides(215 nm). Purified MBP-hNaa30p (80 nM) was incubated with selected oligopeptides and acetyl CoA (300 μM) in acetylation buffer for 30 minutes at 37°C. The acetylation kinetics was analysed by reverse phase HPLC. V/K is the Vmax/Km (oligopeptides). Error bars indicate S.D. Experiments are performed in triplicates.
Evjenth et al. BMC Proceedings 2009 3(Suppl 6):S5 doi:10.1186/1753-6561-3-S6-S5