Figure 8.

Determination of Km and Vmax for acetyl CoA, based on the generation of acetylated ₁MLAL-RRR₂₄ oligopeptide. Purified MBP-hNaa30p (80 nM) was incubated with ₁MLAL-RRR₂₄ oligopeptide at saturated levels and varying concentrations of acetyl CoA (4 – 40 μM) in acetylation buffer for 30 minutes at 37°C. A; Non linear regression analysis of the dose dependent curve generated based on the analysis of the acetylated oligopeptide absorption signal at 215 nm. The coefficient of determination (R²) is given above the plot. B; Hanes-Woolf plot of the dose dependent acetylation signal.