Reverse phase HPLC absorbance profile at 215 nm for the separation of acetylated and non-acetylated peptides. A; The oligopeptide 1MLGTE-RRR24 (200 μM) was incubated with acetyl CoA (300 μM) and purified MBP-hNaa30p (80 nM) in acetylation buffer for 60 minutes at 37°C. Samples were collected at indicated time points and analysed with reverse phase HPLC. The resulting absorbance profile at 215 nm indicate good separation of unacetylated ('a') and acetylated oligopeptides ('b'). B; An expanded version of the absorbance profile for the formation of acetylated oligopeptide. A clear time dependent increase in the absorption signal is observed.
Evjenth et al. BMC Proceedings 2009 3(Suppl 6):S5 doi:10.1186/1753-6561-3-S6-S5