Table 2

Characterization of hNatB in vitro acetylating sequences.

PEPTIDE

CPM


No-peptide

219

Tropomyosin-1

5290

Tropomyosin-1 MA

283

Tropomyosin-1 AD

1724

Tropomyosin-1 AA

221

β-actin

4022

β-actin MA

3760

β-actin AD

2678

β-actin AA

2951


To determine the relevance of the first two amino acids there were used tropomyosin-1 and β-actin aminoterminal peptides (20 amino acids long) as well as modifications of these peptides with the first (AD), second (MA) or both (AA) amino acids changed to an alanine in a NAT assay with immunopurified hNatB complexes. The results are representative of at least two independent experiments.

Ametzazurra et al. BMC Proceedings 2009 3(Suppl 6):S4   doi:10.1186/1753-6561-3-S6-S4

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