Effects on HepG2 and Hep3B p53 expression and cell death after siRNA mediated hNAT5/hNAT3 knockdown. A. Western Blot of Mdm2, p53, Bcl-2 and p21(WAF/CIP1) 24, 48 and 72 hours after infection using GAPDH as loading control. B. Apoptotic cell death quantification using TUNEL technology after hNaa20p expression inhibition for 48 hours and incubated with the proteasome inhibitor MG132 for additional 8 hours. Representative experiment of at least three independent experiments.
Ametzazurra et al. BMC Proceedings 2009 3(Suppl 6):S4 doi:10.1186/1753-6561-3-S6-S4