Figure 1.

Effects of siRNA mediated hNAA20 expression inhibition in HepG2 and Hep3B cellular proliferation. A. Western Blot analysis of hNaa20p in uninfected or infected cells with adenoviruses which express siRNA2 or control siRNA (siRNA350) for 24, 48 and 72 hours. B. Cellular proliferation quantification after siRNA mediated hNAA20 knockdown for 33, 48 and 72 hours measured as the percentage of cells that incorporate BrdU. Representative experiment of at least three independent experiments. C. Cell cycle flow cytometry analysis of control and hNAA20 downregulated expression in HepG2 and Hep3B cells 72 hours after adenovirus infection. The data represent the mean value and standard deviation of the cell percentage present in each phase of the cell cycle from four independent experiments.