Email updates

Keep up to date with the latest news and content from BMC Systems Biology and BioMed Central.

Open Access Highly Accessed Research article

Identification of core T cell network based on immunome interactome

Gabriel N Teku1, Csaba Ortutay23 and Mauno Vihinen123*

Author Affiliations

1 Department of Experimental Medical Science, Lund University, Lund, Sweden

2 Institute of Biomedical Technology, University of Tampere, Tampere, Finland

3 BioMediTech, University of Tampere, Tampere, Finland

For all author emails, please log on.

BMC Systems Biology 2014, 8:17  doi:10.1186/1752-0509-8-17

Published: 15 February 2014

Abstract

Background

Data-driven studies on the dynamics of reconstructed protein-protein interaction (PPI) networks facilitate investigation and identification of proteins important for particular processes or diseases and reduces time and costs of experimental verification. Modeling the dynamics of very large PPI networks is computationally costly.

Results

To circumvent this problem, we created a link-weighted human immunome interactome and performed filtering. We reconstructed the immunome interactome and weighed the links using jackknife gene expression correlation of integrated, time course gene expression data. Statistical significance of the links was computed using the Global Statistical Significance (GloSS) filtering algorithm. P-values from GloSS were computed for the integrated, time course gene expression data. We filtered the immunome interactome to identify core components of the T cell PPI network (TPPIN). The interconnectedness of the major pathways for T cell survival and response, including the T cell receptor, MAPK and JAK-STAT pathways, are maintained in the TPPIN network. The obtained TPPIN network is supported both by Gene Ontology term enrichment analysis along with study of essential genes enrichment.

Conclusions

By integrating gene expression data to the immunome interactome and using a weighted network filtering method, we identified the T cell PPI immune response network. This network reveals the most central and crucial network in T cells. The approach is general and applicable to any dataset that contains sufficient information.

Keywords:
Protein-protein interaction; Network; Filtering; T cell; TPPIN; Signaling; PPI