The expression ratios of 5p arm miRNA to 3p arm miRNA. In order to avoid extreme values caused by low expression levels, we examined only 49 pre-miRNAs whose read counts of 5p arm and 3p arm were 5 in TPM (transcript per million) unit. The cutoff of significant difference of expression ratios between libraries is 1.5 fold change. (a) The expression ratios of 5p arm to 3p arm were averaged from three normal and two HBV(+)HCC libraries. (b,c,d) The N1, N2, N3, T1 and T2 denoted SRX018957, SRX018958, SRX018959, SRX018965 and SRX018967 libraries, respectively. So, NIT1 denoted the comparison in the NT pair composed of SRX018957 and SRX018965.
Li et al. BMC Systems Biology 2012 6(Suppl 2):S14 doi:10.1186/1752-0509-6-S2-S14