|Comparison of experimental with predicted exometabolome fluxes, at 85% maximal biomass level|
|Experiment||Predicted: Gene expression||Predicted: Standard FBA||Predicted: Fitted FBA||Predicted: GIMME||Predicted: iMAT|
Fluxes are reported in units of mmoles/hr/g dry weight (DW), and are scaled by measured glucose uptake flux [see additional file – exometabolomics.xls].
Predicted fluxes are given for the gene expression constrained approach, introduced here, and the standard FBA method that relies upon maximisation of biomass. As standard FBA generates a number of feasible solutions, the “best” solution (the one which minimises the taxicab distance between prediction and data) is reported as “Fitted FBA”. Additionally, the existing algorithms GIMME  and iMAT  are applied, using the same model and gene expression data as was used to generate the gene expression constrained results.
Lee et al.
Lee et al. BMC Systems Biology 2012 6:73 doi:10.1186/1752-0509-6-73