Step 1, both miRNA and mRNA microarray tests for identifying significantly dysregulated miRNAs and mRNAs in colorectal cancer. Step 2, Pearson correlation analysis for anti-correlationship of dysregulated miRNAs and mRNAs. Step 3, TargetScan and Mirnada predicting the target-relationship miRNA-mRNA pairs.
Fu et al. BMC Systems Biology 2012 6:68 doi:10.1186/1752-0509-6-68