Table 2 |
|||
Multiple regression results predicting plasticity | |||
Factor | Subtract Factor from Best Model Predicting Plasticity | Factor in Isolation | |
Y2H PPI | R^{2} | 0.0191^{1} | 0.0194 |
p | *** | *** | |
ACMS PPI | R^{2} | ns | 0.0040 |
p | - | * | |
TATA Box (+/−) | R^{2} | 0.0242^{2} | 0.0445 |
p | *** | *** | |
Self Interaction (+/−) | R^{2} | 0.0087^{3} | 0.0015 |
p | ** | ns | |
Gene Essentiality (+/−) | R^{2} | ns | 0.0005 |
p | - | ns | |
Noise | R^{2} | 0.05344^{4} | 0.0781 |
p | *** | *** | |
Noise if TATA(+) | R^{2} | 0.0315 | 0.0449 |
slope | 167.24 | 0.9949 | |
p | *** | *** | |
Noise if TATA(−) | R^{2} | 0.0224 | 0.0438 |
slope | 70.444 | −0.9858 | |
p | *** | *** | |
TATA × Noise | R^{2} | 0.0085 | - |
p | ** | - | |
Y2H if Self | R^{2} | 0.0123 | 0.0004 |
slope | −0.3145 | −0.0221 | |
p | *** | ns | |
Y2H if Non-self | R^{2} | 0.0070 | 0.0154 |
slope | −0.0839 | −0.1113 | |
p | ** | *** | |
Y2H × Self Interaction | R^{2} | 0.0059 | - |
p | ** | - |
^{1} removed Y2H if Self and Y2H if Non-self.
^{2} also removed Noise if TATA(+), Noise if TATA(−) and restored Noise.
^{3} also removed Y2H if Self and Y2H if Non-self and restored Y2H PPI.
^{4} removed Noise if TATA(+) and Noise if TATA(−).
After extensive model building, we found that high plasticity is predicted by low stickiness (low Y2H degree), presence of a TATA box, ability to bind itself, and high noise. The TATA × noise and self-interaction × Y2H interaction terms are also statistically significant. To provide greater insight, we transformed the interaction terms as described in the Table 1 legend. R^{2} values are shown for each predicting factor in isolation (last column), as well as, more importantly, for the reduction in the total coefficient of determination R^{2} when the factor is removed from the best model. Sometimes, as indicated in the footnotes, this involved removing multiple terms and reversing the interaction factor transformation to get a biologically interpretable result. “ns” indicates p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.
Brettner and Masel
Brettner and Masel BMC Systems Biology 2012 6:128 doi:10.1186/1752-0509-6-128