Additional file 3.

Definition and overview of objectives and constraints for simulation of metabolic and physiological functions of the cardiomyocyte. To ensure consistency and full functionality of the metabolic network, we performed a critical testing of physiological functions based on knowledge of the cardiac metabolism by using flux balance analysis. The table lists all objectives and applied constraints as used in the optimization problem. Furthermore, constraints as used in functional pruning of the network are given. Abbreviations for constraints as used in simulations with FASIMU software are as follows: (+), secretion of the metabolite is allowed or the metabolite is product; (-), uptake of the metabolite is allowed or metabolite is substrate and (=), secretion and uptake of the metabolite is allowed or metabolites is either product or substrate.

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Karlst├Ądt et al. BMC Systems Biology 2012 6:114   doi:10.1186/1752-0509-6-114