Analysis of C1qa by a luciferase assay
Plasmids were constructed that carried the pGL4 C1qa promoter-luciferase and MafB and GATA-1 motifs. RAW264.7 cells were transfected to express each construct and the effects on luciferase reporter activity were studied. The Single GATA-1 in the C1qa promoter had little influence. Inducing MafB increased C1qa promoter driven luciferase activity. This activity was inhibited by GATA-1.
Morita et al. BMC Systems Biology 2011 5(Suppl 2):S7 doi:10.1186/1752-0509-5-S2-S7