Summary of approach. (A) The proposed approach uses input-output data to generate mathematical models capable of predicting the cellular responses to input combinations. The models enable using other mathematical tools for analyzing the cellular responses and for selecting the appropriate combinations of the input signal to drive the system to respond favorably. (B) The desired drugs are combined in certain concentrations and a few combinations are chosen and evaluated experimentally. A predictive mathematical model is generated that can predict the response to all possible combinations. The model can be used to analyze and predict drug interactions and their effects on the observed cellular response and can also be used to determine effective combinations.
Al-Shyoukh et al. BMC Systems Biology 2011 5:88 doi:10.1186/1752-0509-5-88