Predictions of the model can distinguish between different scenarios. Two possible scenarios can result in a constant initiation threshold for the model. In the first the binding affinity of DnaA-ATP to its repressor sites decreases with increasing cell doubling time, and in the second the RIDA rate increases with cell doubling time. In both scenarios kA (the basal transcription rate) must decrease with increasing cell doubling time.
Grant et al. BMC Systems Biology 2011 5:201 doi:10.1186/1752-0509-5-201