Results of the ChiP and qPCR experiments. His-tagged PdhR was crosslinked to DNA and purified. The co-precipitated DNA was analysed for the frequency of copies, which contain the putative binding sites of PdhR. The operator fragment of the not controlled ptsG gene was set as 1. The amount of DNA fragments which contain the known self-regulating pdhR binding site of PdhR was enriched to 11.6 times more copies compared to the negative control. The glcD and mraZ operator fragments occur to be 1.8 and 1.7 more abundant than the control fragment. The metB DNA fragment was detected with the same frequency as the control fragment and the ynfM binding site was observed to be less precipitated than the negative control. The data are mean values with standard deviations of three experiments. The statistical significance of the binding of PdhR to pdhR, glcD and mraZ if compared to the control by a One-Way-ANOVA test is indicated by asterisks (***: p-value < 0.001, **: p-value < 0.01).
Göhler et al. BMC Systems Biology 2011 5:197 doi:10.1186/1752-0509-5-197