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Modeling RNA interference in mammalian cells

Giulia Cuccato1, Athanasios Polynikis2, Velia Siciliano1, Mafalda Graziano1, Mario di Bernardo23 and Diego di Bernardo13*

Author Affiliations

1 Telethon Institute of Genetics and Medicine (TIGEM), Naples, Italy

2 Department of Engineering Mathematics, University of Bristol, Bristol, UK

3 Department of Computer and Systems Engineering, University of Naples Federico II, Naples, Italy

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BMC Systems Biology 2011, 5:19  doi:10.1186/1752-0509-5-19

Published: 27 January 2011

Additional files

Additional file 1:

Supplementary material for Modeling RNA interference in mammalian cells. Results and fitting of in vitro experiments on hamster ovary cell line (CHO) constitutively expressing tTA protein. We measured mRNA levels, by quantitative Real-Time PCR for a large range of concentrations of siRNA oligomers, from 0.001 pmol to 200 pmol (total concentration). The amounts of transfected siRNA oligomers were: 0, 0.001, 0.01, 0.05, 0.1, 0.5, 1.0, 10.0, 20.0, 40.0, 60.0, 80.0, 100.0 and 200.0 pmol in a total of 2 mL of medium (so the final concentrations of siRNA oligomers were 5 × 10-4, 5 × 10-3, 2.5 × 10-2, 5 × 10-2, 2.5 × 10-1, 5 × 10-1, 5.0, 10.0, 20.0, 30.0, 40.0, 50.0, and 100 nM respectively). Each experiment was performed in biological triplicates, and the resulting standard deviations are computed and reported in each graph. In Additional file 1 Table A1, numerical fitting results and predicted error for the four models, in Additional file 1 Figure A1, graphic representation of the numerical fitting.

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