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Open Access Research article

Systemic remodeling of the redox regulatory network due to RNAi perturbations of glutaredoxin 1, thioredoxin 1, and glucose-6-phosphate dehydrogenase

Linda E Kippner1, Nnenna A Finn12, Shreya Shukla13 and Melissa L Kemp1*

Author Affiliations

1 The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA

2 Department of Medicine, Division of Cardiology, Emory University, Atlanta, GA, USA

3 Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada

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BMC Systems Biology 2011, 5:164  doi:10.1186/1752-0509-5-164

Published: 13 October 2011

Additional files

Additional file 1:

Significant changes in mRNA expression levels in shRNA and pLKO cells lines. Significant changes (p < 0.05) in mRNA expression levels in shRNA cell lines and pLKO control cells compared to wild-type Jurkat cells, and in shRNA cells and wild-type cells compared to pLKO cells. Names in black represent targets that were expressed in all five of our cells lines, while names in red represent mRNA targets that were not expressed (Ct ≥ 35) in any of our cells lines.

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Additional file 2:

Description of computational model used for simulations in figures 5and 6. Further details of model description and sources for parameter values can be found in Adimora NJ, Jones DP, Kemp ML. "A model of redox kinetics implicates the thiol proteome in cellular hydrogen peroxide responses." Antioxid Redox Signal 2010, 13:731-743. Each cell line represented in the simulations was modified by adjusting the subset of parameters listed in Table 2 of the main text.

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