Open Access Research article

In vivo kinetics of transcription initiation of the lar promoter in Escherichia coli. Evidence for a sequential mechanism with two rate-limiting steps

Meenakshisundaram Kandhavelu1, Henrik Mannerström1, Abhishekh Gupta1, Antti Häkkinen1, Jason Lloyd-Price1, Olli Yli-Harja12 and Andre S Ribeiro1*

Author Affiliations

1 Laboratory of Biosystem Dynamics, Computational Systems Biology Research Group, Department of Signal Processing, Tampere University of Technology, 33101 Tampere, Finland

2 Institute for Systems Biology, 1441N 34th St, Seattle, WA, 98103-8904, USA

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BMC Systems Biology 2011, 5:149  doi:10.1186/1752-0509-5-149

Published: 25 September 2011

Additional files

Additional file 1:

Example of a movie generated from temporal images of a cell. Images were taken approximately 7 min after induction, one every minute, for approximately 2 hours. The cell was induced with 0.01 mM of IPTG and 0.067 mM of Arabinose. The cell identification number and the time (s) when the frame was captured are shown in the top right and left corners, respectively.

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Additional file 2:

Supplementary information. Supplementary information: qPCR analysis of the target RNA; image analysis and cell segmentation, detection and counting of mRNA in cells; analyses of the intervals between production events assuming an ON-OFF mechanism of RNA production; measurements of RNA numbers under full induction.

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