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Open Access Research article

Computational modelling elucidates the mechanism of ciliary regulation in health and disease

Nikolay V Kotov12, Declan G Bates3, Antonina N Gizatullina2, Bulat Gilaziev2, Rustem N Khairullin4, Michael ZQ Chen56, Ignat Drozdov7, Yoshinori Umezawa8, Christian Hundhausen8, Alexey Aleksandrov9, Xing-gang Yan10, Sarah K Spurgeon10, C Mark Smales1 and Najl V Valeyev1*

Author Affiliations

1 Centre for Molecular Processing, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK

2 Biophysics & Bionics Lab, Department of Physics, Kazan State University, Kazan 420008, Russia

3 Centre for Systems, Dynamics and Control, College of Engineering, Mathematics and Physical Sciences, University of Exeter, Harrison Building, North Park Road, Exeter EX4 4QF, UK

4 Inter-regional Diagnostic Centre, Karbisheva-12A, Kazan 420101, Russia

5 Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong

6 School of Automation, Nanjing University of Science and Technology, 200 Xiao Ling Wei, Nanjing 210094, P. R. China

7 Centre for Bioinformatics, Department of Computer Science, School of Physical Sciences & Engineering, King's College London, Strand, London WC2R 2LS, UK

8 St. John's Institute of Dermatology, King's College London, 9th Floor Tower Wing, Guy's Hospital, Great Maze Pond, SE1 9RT London, UK

9 Laboratoire de Biochimie, CNRS UMR7654, Department of Biology, Ecole Polytechnique, 91128 Palaiseau, France

10 School of Engineering and Digital Arts, University of Kent, Canterbury, Kent CT2 7NT, UK

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BMC Systems Biology 2011, 5:143  doi:10.1186/1752-0509-5-143

Published: 15 September 2011

Abstract

Background

Ciliary dysfunction leads to a number of human pathologies, including primary ciliary dyskinesia, nephronophthisis, situs inversus pathology or infertility. The mechanism of cilia beating regulation is complex and despite extensive experimental characterization remains poorly understood. We develop a detailed systems model for calcium, membrane potential and cyclic nucleotide-dependent ciliary motility regulation.

Results

The model describes the intimate relationship between calcium and potassium ionic concentrations inside and outside of cilia with membrane voltage and, for the first time, describes a novel type of ciliary excitability which plays the major role in ciliary movement regulation. Our model describes a mechanism that allows ciliary excitation to be robust over a wide physiological range of extracellular ionic concentrations. The model predicts the existence of several dynamic modes of ciliary regulation, such as the generation of intraciliary Ca2+ spike with amplitude proportional to the degree of membrane depolarization, the ability to maintain stable oscillations, monostable multivibrator regimes, all of which are initiated by variability in ionic concentrations that translate into altered membrane voltage.

Conclusions

Computational investigation of the model offers several new insights into the underlying molecular mechanisms of ciliary pathologies. According to our analysis, the reported dynamic regulatory modes can be a physiological reaction to alterations in the extracellular environment. However, modification of the dynamic modes, as a result of genetic mutations or environmental conditions, can cause a life threatening pathology.