Table 4

Ranked pathways for biosynthesis of taxol in S. cerevisiae

EC number

product

cost

ρ1

ρ2


2.3.1.- *

C07394

1.34

X

X


2.3.1.167

C11900

1.17

X

X


2.3.1.166

C11700

1.07

X

X


1.14.14.1*

C11899

1.78

X

X


1.14.13.76

C11898

5.64

-

X


1.14.13.77

C11897

5.62

X

-


2.3.1.162

C11896

1.06

-

X


1.14.99.37

C11895

5.62

X

X


4.2.3.17

C11894

1.28

X

X


2.5.1.29

C00353

0.69

X

X


vc(ρ)

0.094

0.093


W(ρ)

18.64

19.72


Each row corresponds to the insertion of one enzyme in the pathway in order to produce the given intermediate product in the second column. The estimated cost is given in the third column 3. The last two rows provide the estimate of maximum flux vc(ρ) for the pathway, and the total cost W(ρ) according to Equation 3. C07394: Paclitaxel; C11900: Baccatin; C11700: 10-Deacetylbaccatin; C11899: 10-Deacetyl-2-debenzoylbaccatin; C11898: Taxa-4(20),11(12)-dien-5α-acetoxy-10β-ol; C11897: Taxa-4(20),11(12)-dien-5α,13α-diol; C11896: Taxa-4(20),11(12)-dien-5α-yl; C11895: Taxa-4(20),11(12)-dien-5α-ol; C11894: Taxa-4(5),11(12)-diene; C00353: Geranylgeranyl. Starred EC numbers correspond to putative enzymes.

Carbonell et al. BMC Systems Biology 2011 5:122   doi:10.1186/1752-0509-5-122

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