Flux solution space of wild-type and mutants (ΔprpB, ΔprpD, or ΔacnM) of R. eutropha. Based on the flux solution space of wild-type, 2-methylcitric acid is non-growth associated metabolite when the cell growth rate is maximized. After the in silico single knockout of each gene, 2-methylcitric acid is changed to the growth-associated metabolite according to the flux solution space of the mutants. The x and y axes indicate the relative flux (%) of 2-methylcitric acid production rate and cell growth rate that are normalized to the maximum values of 2-methylcitric acid production rate and cell growth rate in the wild-type of R. eutropha, respectively.
Park et al. BMC Systems Biology 2011 5:101 doi:10.1186/1752-0509-5-101