Figure 2.

Effects on poly[R-(-)-3hydroxybutyrate] (PHB) production for different pH values and carbon/nitrogen source uptake ratios. (A) Genes, enzymes, and reactions for the biosynthesis of polyhydroxyalkanoate (PHA). (B) PHB production yield for different pH values, which are pH 6, 7, and 8. The PHB content (%wt) is defined as the percentage of PHB concentration (g∙L^-1) to cell concentration (g∙L^-1). The culture medium is the MR minimal medium with D-fructose. (C) Mesh plots graph 3D data as a continuous surface for nitrogen uptake rate, PHB production rate, and growth rate. (D) Predicted PHB production rate by limiting nitrogen uptake rate for the maximization of growth rate.