Expression of putative effector proteins under in vitro and ex vivo conditions. Candidate proteins and two SPI-2 proteins (SseA and SsaN) were labeled with 2HA or CyaA' and their expression was examined under in vitro (LB log phase and AMM1) and ex vivo (RAW264.7 cells infected with HA or CyaA'-tagged strains) conditions. In order to compare protein expressions across conditions, the signal intensity in Western blot analyses (Additional file 7, Figure S3) was quantified via ImageJ software based analysis (http://rsb.info.nih.gov/ij/ webcite). Protein levels in LB log phase, AMM1, and macrophages (18 h post-infection) conditions were normalized using DnaK levels and the expression fold was compared in three conditions.
Yoon et al. BMC Systems Biology 2011 5:100 doi:10.1186/1752-0509-5-100