Open Access Highly Accessed Research article

Systems analysis of multiple regulator perturbations allows discovery of virulence factors in Salmonella

Hyunjin Yoon1, Charles Ansong2, Jason E McDermott2, Marina Gritsenko2, Richard D Smith2, Fred Heffron1 and Joshua N Adkins2*

Author Affiliations

1 Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon 97239, USA

2 Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA

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BMC Systems Biology 2011, 5:100  doi:10.1186/1752-0509-5-100

Published: 28 June 2011

Additional files

Additional file 1:

Table S1. Proteins quantified by mass spectrometry.

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Additional file 2:

Methods S1.

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Additional file 3:

Figure S1. Integration of networks inferred from transcriptomics and proteomics improves enrichment of genes essential for virulence in Salmonella.

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Additional file 4:

Table S2. Topological enrichment in protein association and integrated networks.

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Additional file 5:

Table S3. Network topology of integrated network.

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Figure S2. Coordinate regulation of proteomics-identified novel effectors

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Additional file 7:

Figure S3. Expression of candidate proteins under in vitro and ex vivo conditions.

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Additional file 8:

Figure S4. Sequence alignment of pagJ, pagK, and STM2585A.

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Figure S5. Translocation of SseJ into the macrophage cytosol.

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Additional file 10:

Figure S6. Translocation of SipA and SseJ in macrophages infected with ΔinvA and ΔssaK strains.

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Additional file 11:

Figure S7. Effects of SPI-2 TTSS on the transcription of srfN, and pagJ/pagK1/pagK2 inside macrophages.

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Additional file 12:

Figure S8. Effects of PhoP/PhoQ and SsrA/SsrB on the transcription of srfN and pag genes.

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Additional file 13:

Table S4. Primers used in strains construction.

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Additional file 14:

Table S5. Primers used in qRT-PCR.

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