Research article
Systems analysis of multiple regulator perturbations allows discovery of virulence factors in Salmonella
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* Corresponding author: Joshua N Adkins Joshua.adkins@pnl.gov
- † Equal contributors
1 Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon 97239, USA
2 Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA
BMC Systems Biology 2011, 5:100 doi:10.1186/1752-0509-5-100
Published: 28 June 2011Additional files
Additional file 1:
Table S1. Proteins quantified by mass spectrometry.
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Additional file 2:
Methods S1.
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Additional file 3:
Figure S1. Integration of networks inferred from transcriptomics and proteomics improves enrichment of genes essential for virulence in Salmonella.
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Additional file 4:
Table S2. Topological enrichment in protein association and integrated networks.
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Additional file 5:
Table S3. Network topology of integrated network.
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Additional file 6:
Figure S2. Coordinate regulation of proteomics-identified novel effectors
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Additional file 7:
Figure S3. Expression of candidate proteins under in vitro and ex vivo conditions.
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Additional file 8:
Figure S4. Sequence alignment of pagJ, pagK, and STM2585A.
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Additional file 9:
Figure S5. Translocation of SseJ into the macrophage cytosol.
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Additional file 10:
Figure S6. Translocation of SipA and SseJ in macrophages infected with ΔinvA and ΔssaK strains.
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Additional file 11:
Figure S7. Effects of SPI-2 TTSS on the transcription of srfN, and pagJ/pagK1/pagK2 inside macrophages.
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Additional file 12:
Figure S8. Effects of PhoP/PhoQ and SsrA/SsrB on the transcription of srfN and pag genes.
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Additional file 13:
Table S4. Primers used in strains construction.
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Additional file 14:
Table S5. Primers used in qRT-PCR.
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