Table 3 |
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|
Changes in enzyme activities following PQ stressa |
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|
Enzymes |
Metabolic steps |
Activity normal conditions |
Activity paraquat treated |
PQ/normal Ratios |
|
|
||||
|
IDH |
icd |
0.301 ± 0.009 |
0.155 ± 0.010 |
0.51 |
|
AKGDH |
akd |
0.191 ± 0.008 |
0.090 ± 0.015 |
0.47 |
|
MDH |
mdh |
0.106 ± 0.003 |
0.303 ± 0.013 |
2.86 |
|
G6PDH |
gdh |
0.428 ± 0.000 |
1.219 ± 0.011 |
2.85 |
|
PGI |
hxi |
0.207 ± 0.003 |
0.297 ± 0.007 |
1.44 |
|
ICL |
gs1 |
0.787 ± 0.012 |
1.506 ± 0.004 |
1.91 |
|
ENO |
eno |
0.072 ± 0.012 |
0.072 ± 0.013 |
1.00 |
|
THD |
- |
0.122 ± 0.005 |
0.181 ± 0.003 |
1.48 |
|
|
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|
aEnzyme activities in cell extracts of control or paraquat-treated wildtype E. coli JM101 grown on glucose at D = 0.17 h-1 under aerobic chemostat conditions. Ratio calculation was based on control conditions (wildtype aerobic growth on glucose). The unit of enzyme activity is μmol min-1 (mg protein) -1. All measurements were performed in triplicate. Data are expressed as means ± S.D. IDH: isocitrate dehydrogenase NADP-dependent, AKGDH: α-ketoglutaric acid dehydrogenase, PGI: glucose phosphate isomerase, MDH: malate dehydrogenase, G6PDH: glucose-6-phosphate dehydrogenase, ICL: isocitrate lyase, ENO: enolase, THD: transhydrogenase. |
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|
Rui et al. BMC Systems Biology 2010 4:122 doi:10.1186/1752-0509-4-122 |
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