Table 4

Top ranking predicted essential reactions

Enzyme name

Compartment

Pathway

EC

Super-Target

Score


adenylate cyclase

apicoplast

Purine metabolism

4.6.1.1

x

4

fumarase

cytosol

Citrate cycle

4.2.1.2

x

4

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

apicoplast

Biosynthesis of isopentenyl diphosphate

2.7.7.60

x

4

4-hydroxy-3-methylbut-2-enoyl-diphosphate synthase

apicoplast

Biosynthesis of isopentenyl diphosphate

1.17.4.3

3

CDP-ME kinase

apicoplast

Biosynthesis of isopentenyl diphosphate

2.7.1.148

3

pantetheine-phosphate adenylyltransferase

cytosol

CoA biosynthesis

2.7.7.3

3

pyridoxal 5-phosphate synthase

cytosol

Vitamin B6 metabolism

3

amino acid transporter

Cytosol ↔ apicoplast

Transport

3

geranyl-diphosphate synthase

cytosol

Terpenoid biosynthesis

2.5.1.1

x

2

farnesyl-diphosphate synthase

cytosol

Terpenoid biosynthesis

2.5.1.10

x

2

2-Octaprenylphenol hydroxylase

mitochondrion

Ubiquinone biosynthesis

1.14.13.-

x

2

ubiquinone biosynthesis methyltransferase

mitochondrion

Ubiquinone biosynthesis

2.1.1.-

x

2

uroporphyrinogen decarboxylase

apicoplast

Porphyrin metabolism

4.1.1.37

x

2

coproporphyrinogen oxidase

apicoplast

Porphyrin metabolism

1.3.3.3

x

2

UMP-CMP kinase

apicoplast

Pyrimidine metabolism

2.7.4.14

x

2

aspartate carbamoyltransferase

cytosol

Pyrimidine metabolism

2.1.3.2

x

2

orotate phosphoribosyltransferase

cytosol

Pyrimidine metabolism

2.4.2.10

x

2

cysteine desulfurase

apicoplast/mitochondrion

Fe-S-protein biogenesis

2.8.1.7

x

2

tRNA ligase (Ile, Lys, Met, Trp, Tyr)

cytosol/apicoplast/mitochondrion

Aminoacyl-tRNA biosynthesis

6.1.1.1/6.1.1.2/6.1.1.5/6.1.1.6/6.1.1.10

x

2

glutathione synthase

cytosol

Glutathione metabolism

6.3.2.3

x

2

riboflavin kinase

cytosol

Riboflavin metabolism

2.7.1.26

x

2

glucosamine 6-phosphate synthase

apicoplast

Aminosugars metabolism

2.6.1.16

x

2

mannose-6-phosphate isomerase

cytosol

Fructose and mannose metabolism

5.3.1.8

x

2

cytochrome-c oxidase

mitochondrion

Oxidative phosphorylation

1.9.3.1

x

2

choline-phosphate cytidylyltransferase

cytosol

Glycerophospholipid metabolism

2.7.7.15

x

2

deoxyhypusine synthase

cytosol

Activation of eiF5A

2.5.1.46

x

2


Huthmacher et al. BMC Systems Biology 2010 4:120   doi:10.1186/1752-0509-4-120

Open Data