Table 4 |
|||||
|
Top ranking predicted essential reactions |
|||||
|
Enzyme name |
Compartment |
Pathway |
EC |
Super-Target |
Score |
|
|
|||||
|
adenylate cyclase |
apicoplast |
Purine metabolism |
4.6.1.1 |
x |
4 |
|
fumarase |
cytosol |
Citrate cycle |
4.2.1.2 |
x |
4 |
|
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase |
apicoplast |
Biosynthesis of isopentenyl diphosphate |
2.7.7.60 |
x |
4 |
|
4-hydroxy-3-methylbut-2-enoyl-diphosphate synthase |
apicoplast |
Biosynthesis of isopentenyl diphosphate |
1.17.4.3 |
3 |
|
|
CDP-ME kinase |
apicoplast |
Biosynthesis of isopentenyl diphosphate |
2.7.1.148 |
3 |
|
|
pantetheine-phosphate adenylyltransferase |
cytosol |
CoA biosynthesis |
2.7.7.3 |
3 |
|
|
pyridoxal 5-phosphate synthase |
cytosol |
Vitamin B6 metabolism |
3 |
||
|
amino acid transporter |
Cytosol ↔ apicoplast |
Transport |
3 |
||
|
geranyl-diphosphate synthase |
cytosol |
Terpenoid biosynthesis |
2.5.1.1 |
x |
2 |
|
farnesyl-diphosphate synthase |
cytosol |
Terpenoid biosynthesis |
2.5.1.10 |
x |
2 |
|
2-Octaprenylphenol hydroxylase |
mitochondrion |
Ubiquinone biosynthesis |
1.14.13.- |
x |
2 |
|
ubiquinone biosynthesis methyltransferase |
mitochondrion |
Ubiquinone biosynthesis |
2.1.1.- |
x |
2 |
|
uroporphyrinogen decarboxylase |
apicoplast |
Porphyrin metabolism |
4.1.1.37 |
x |
2 |
|
coproporphyrinogen oxidase |
apicoplast |
Porphyrin metabolism |
1.3.3.3 |
x |
2 |
|
UMP-CMP kinase |
apicoplast |
Pyrimidine metabolism |
2.7.4.14 |
x |
2 |
|
aspartate carbamoyltransferase |
cytosol |
Pyrimidine metabolism |
2.1.3.2 |
x |
2 |
|
orotate phosphoribosyltransferase |
cytosol |
Pyrimidine metabolism |
2.4.2.10 |
x |
2 |
|
cysteine desulfurase |
apicoplast/mitochondrion |
Fe-S-protein biogenesis |
2.8.1.7 |
x |
2 |
|
tRNA ligase (Ile, Lys, Met, Trp, Tyr) |
cytosol/apicoplast/mitochondrion |
Aminoacyl-tRNA biosynthesis |
6.1.1.1/6.1.1.2/6.1.1.5/6.1.1.6/6.1.1.10 |
x |
2 |
|
glutathione synthase |
cytosol |
Glutathione metabolism |
6.3.2.3 |
x |
2 |
|
riboflavin kinase |
cytosol |
Riboflavin metabolism |
2.7.1.26 |
x |
2 |
|
glucosamine 6-phosphate synthase |
apicoplast |
Aminosugars metabolism |
2.6.1.16 |
x |
2 |
|
mannose-6-phosphate isomerase |
cytosol |
Fructose and mannose metabolism |
5.3.1.8 |
x |
2 |
|
cytochrome-c oxidase |
mitochondrion |
Oxidative phosphorylation |
1.9.3.1 |
x |
2 |
|
choline-phosphate cytidylyltransferase |
cytosol |
Glycerophospholipid metabolism |
2.7.7.15 |
x |
2 |
|
deoxyhypusine synthase |
cytosol |
Activation of eiF5A |
2.5.1.46 |
x |
2 |
|
|
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|
Huthmacher et al. BMC Systems Biology 2010 4:120 doi:10.1186/1752-0509-4-120 |
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