Pathway differences between adapted and naïveflies. A) Fluxes through key reactions at a common oxygen uptake. Compared with naïve flies, simulations of adapted flies showed lower glycolysis, pyruvate carboxylase, GPDH, and Complex II activity, and higher fluxes through Complex I. All tests between the adapted group and the two naïve groups had p < 0.05, except for LDH, which is shown for comparison only. Error bars indicate standard deviation of 100 simulations incorporating the variances of NMR measurements. B) Cartoon of differences in fluxes in simulations of adapted flies versus naïve controls. In naïve flies, oxalacetate production in the NMR profiles drives increased activity of glycolysis, pyruvate carboxylase, and Complex II of the electron transport chain. Abbreviations: PYK - pyruvate kinase, ACS - acetyl-CoA synthase, ALAT - alanine transaminase (cytosolic), LDH - lactate dehydrogenase, PC - pyruvate carboxylase, PDH - pyruvate dehydrogenase, CS - citrate synthase, SuccDH - succinyl CoA dehydrogenase (TCA cycle), MDH - malate dehydrogenase (mitochondrial), GPDH - glycerol-3-phosphate dehydrogenase shuttle, Complex II - succinyl CoA dehydrogenase (electron transport chain), Complex I - NADH dehydrogenase.
Feala et al. BMC Systems Biology 2009 3:91 doi:10.1186/1752-0509-3-91