Figure 11.

Possibilistic flux estimation for C. glutamicum with uncertainty in the model. The marginal possibility distributions for each flux are depicted in three cases: (a) the model-based constraints are not relaxed (red) (b) the pseudo-steady state assumption is relaxed and NADP/NADPH is allowed to be unbalanced (deep blue), and (c) the pseudo-steady state assumption is relaxed and the three cofactors – NADP/NADPH, FAD/FADH and NAD/NADH – are allowed to be unbalanced (light green). The original distribution of single measurements are depicted with dashed lines. All fluxes in mM/h.