Experimental validation of microarray results by RT-PCR analysis of 10 randomly selected TGF-β/SMAD target genes. The mRNAs of 10 genes from IOSE cells, treated with TGF-β 1, were amplified by RT-PCR and measured by a real-time PCR machine. The fold-change in mRNA expression for each gene was calculated by setting the expression (RT-PCR or microarray) values at 0 hr to 1. The plots are in log2 scale.
Qin et al. BMC Systems Biology 2009 3:73 doi:10.1186/1752-0509-3-73