Experimental validation of ChIP-chip results by ChIP-PCR analysis of 10 randomly selected TGF-β/SMAD target genes. The cross-linked DNA from IOSE cells treated with TGF-β 1 of 10 genes were amplified by ChIP assays and measured by a real-time PCR machine. The ChIP-PCR values were from normalized experimental results from a standard curve, which was derived from total input DNA using the same primers. The plots are in log2 scale.
Qin et al. BMC Systems Biology 2009 3:73 doi:10.1186/1752-0509-3-73