Figure 4.

Prediction of genetic targets for improved lysine production in C. glutamicum based on correlation of flux through metabolic reactions with lysine production flux among the calculated elementary modes: Optimal flux distribution for lysine production in Corynebacterium glutamicum on glucose as obtained from elementary mode analysis (A) and resulting target potential coefficients (B). In the flux map all fluxes are given as relative molar flux normalized to the uptake flux. The data shown display the average fluxes and deviations from the different elementary modes under optimum production conditions. The coloured arrows reflect amplification (green) and deletion/attenuation targets (red). In the heat map, listing the predicted targets, a positive value (green) relates to a reaction, which positively correlates with the production (amplification target), whereas negative correlation (red) displays a deletion/attenuation target. Black colour indicates statistically insignificant values.