BMC Systems Biology

official impact factor 3.57

Open Access Research article

13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose

Paula Jouhten1*, Esa Pitkänen2, Tiina Pakula1, Markku Saloheimo1, Merja Penttilä1 and Hannu Maaheimo1

Author Affiliations

1 VTT Technical Research Centre of Finland, Espoo, Finland

2 Department of Computer Science, University of Helsinki, Helsinki, Finland

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BMC Systems Biology 2009, 3:104 doi:10.1186/1752-0509-3-104

Published: 29 October 2009

Additional files

Additional file 1:

Pathways discovered in ReTrace carbon path analysis. Graphical and tabular representations of amino acid synthesis pathways discovered in ReTrace carbon path analysis [21]. Self-contained web site: unpack zip archive and open index.html with a web browser.

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Additional file 2:

Estimation of subcellular localization of key enzymes by TargetP. TargetP machine learning program [33,34] was used to predict the subcellular localization of key enzymes of amino acid biosynthetic routes in T. reesei. Targeting sequences were predicted for T. reesei sequences that showed the highest homology to the aspartate aminotransferase, acetolactate synthase, serine hydroxymethyltransferase and glycine dehydrogenase in S. cerevisiae.

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