Figure 13.

In (A), the response of the LTD circuit is shown. In (B), the response obtained from HTD is illustrated. In (C), the response obtained prior to optimization of kinetic parameters is shown. Note that this response deviates significantly from the desired response. In (D), the response obtained after optimizing kinetic parameters is shown. Specifically the efficiency of constitutive promoter expressing CRP is decreased 13 fold. In all the figures cubic splines were used to interpolate the observed steady-state expression levels.