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This article is part of the supplement: BioSysBio 2007: Systems Biology, Bioinformatics, Synthetic Biology

Open Access Poster presentation

A quantitative model for the GCN4 translational control in Saccharomyces cerevisiae

Tao You12*, Alistair JP Brown1 and George M Coghill2

Author Affiliations

1 Institute of Medical Science, University of Aberdeen, UK

2 Department of Computing Science, University of Aberdeen, UK

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BMC Systems Biology 2007, 1(Suppl 1):P1  doi:10.1186/1752-0509-1-S1-P1


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1752-0509/1/S1/P1


Published:8 May 2007

© 2007 You et al; licensee BioMed Central Ltd.

Poster presentation

Cells are capable of accommodating the environmental changes by reprogramming gene expression. In the yeast Saccharomyces cerevisiae, under the amino acid deprivation condition, the translational barrier primarily exerted on GCN4 mRNA is alleviated to enhance the production of Gcn4p, which subsequently induces the expression of nearly all genes involved in amino acid biosyntheses. Our Systems Biology project's goal is to construct a dynamic mathematical model that accurately predicts the kinetic behaviour of the GCN Response in S. cerevisiae. Here, we report the mathematical formulations to describe the translational control exerted on the GCN4 mRNA. More importantly, the kinetic parameter estimation for this model indicates differential scanning rates of the 40S ribosomal subunit on the GCN4 mRNA 5' leader sequence under the amino acid replete and starvation conditions. This discovery is speculated to be attributable to the change in relative helicase activities under the two conditions.

References

  1. Hinnebusch AG: Translational regulation of GCN4 and the general amino acid control of yeast.

    Annu Rev Microbiol 2005, 59:407-50.

    doi:10.1146/annurev.micro.59.031805.133833 pmid:16153175.

    PubMed Abstract | Publisher Full Text OpenURL

  2. Grant CM, Miller PF, Hinnebusch AG: Requirements for intercistronic distance and level of eukaryotic initiation factor 2 activity in reinitiation on GCN4 mRNA vary with the downstream cistron.

    Mol Cell Biol 1994, 14(4):2616-28.

    pmid:8139562

    PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  3. Gillespie DT: Exact stochastic simulation of coupled chemical reactions.

    J Phys Chem 1977, 81(25):2340-2361. Publisher Full Text OpenURL

  4. Berthelot K, Muldoon M, Rajkowitsch L, Hughes J, McCarthy JE: Dynamics and processivity of 40S ribosome scanning on mRNA in yeast.

    Mol Microbiol 2004, 51(4):987-1001.

    pmid:14763975.

    PubMed Abstract | Publisher Full Text OpenURL

  5. Runarsson TP, Yao X: Stochastic Ranking for Constrained Evolutionary Optimization.

    IEEE Transactions on Evolutionary Computation 2000, 4(3):284-294. Publisher Full Text OpenURL