Figure 1.

An overview of the hierarchical approach. First, important metabolic changes relevant to a phenotype were identified with discriminant analysis. Second, to identify the transcriptionally altered pathways, gene set enrichment analysis (GSEA) was applied to the cDNA microarray data. Finally, the expression of the enriched gene sets and the metabolic profiles were integrated with a multi-block partial least squares analysis (MBPLS) regression model to identify the genes and gene sets that regulate the metabolic pathways identified in step 1.