Table 1

Targetable phenotypic attributes of leukocytes in vitro: an abridged list
Phenotypic Attribute
 Figure/Table
 Reference
Set A: initially targeted attributes

Jerky stop and go movement
 Figures 5, 6
 [16]
Highly fluctuating rolling velocities
 Figure 7A
 [15]
Larger rolling velocities observed at higher shear rates
 Figures 6A, 8
 [50]
Smaller rolling velocities at higher ligand substrate densities
 Not Shown
 [50]
Set B: additional attributes added to targeted set

ROLLINGa velocities on PSELECTINa match reported values
 Table 6
 [50]
Small number of bonds within the contact zone, e.g., within 2–20
 Table 6
 [19], [20]
Distance-time and velocity-time data for ROLLINGa on aPSELECTIN/VCAM1 are indistinguishable from reported data
 Figures 6, 7, 8
 [15], [16]
Chemokines induce adhesion within seconds
 Figure 10
 [3]
Leukocyte spreading during firm adhesion
 Figure 10
 [51]
Set C: future, targetable attributes

Ligand-Receptor mobility, valency (clustering), and trafficking
 N/A
 [5], [6], [52]
Change in contact surface area with increasing shear force
 N/A
 [25], [53]
Shear threshold effect for selectins (minimum shear required for rolling)
 N/A
 [50]
Effect of inhibitors to signaling molecules on cell arrest (pertussis toxin (PTx)-sensitive G proteins, phosphoinositide 3-kinase, p38 mitogen-activated protein kinase)
 N/A
 [3]
Intracellular calcium mobilization
 N/A
 [3]

During early development of the ISWBCs attention focused primarily on Set A. Once satisfactory simulations began to be achieved, the targeted attributes were expanded to those in Set B. Final validations focused on the combined set.

a We use SMALL CAPS when referring to the in silico components (see Table 3), features, measurements, and events.

Tang et al. BMC Systems Biology 2007 1:14   doi:10.1186/1752-0509-1-14